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A hybrid zone of the genus Ctenomys: a case study in southern Brazil Genet. Mol. Biol.
Castilho,Camila S.; Gava,Adriana; Freitas,Thales R.O. de.
We describe variation at microsatellite loci and the chromosomal polymorphisms of a hybrid population, and hybridizing populations of Ctenomys minutus (the minor tuco-tuco) from the coastal plain of Rio Grande do Sul, southern Brazil. Cytogenetic analysis and a survey of six microsatellite loci included 101 specimens of C. minutus from the parental populations (2n/AN = 42/74 and 48a/76) and their contact zone. Cytogenetic analysis recorded 26 different karyotypes exhibited by 50 individuals from the hybrid population. Of the 26 karyotypes, only 14% presented a parental-like configuration, and none had the combinations of 2n and AN expected for an F1 hybrid. The remaining karyotypes were alternative hybrid forms, with 2n varying from 42 to 46 and AN from 68...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome polymorphism; Subterranean rodent; Karyotypes; Microsatellite.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600014
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Genetic structure and conservation of Mountain Lions in the South-Brazilian Atlantic Rain Forest Genet. Mol. Biol.
Castilho,Camila S.; Marins-Sá,Luiz G.; Benedet,Rodrigo C.; Freitas,Thales R.O..
The Brazilian Atlantic Rain Forest, one of the most endangered ecosystems worldwide, is also among the most important hotspots as regards biodiversity. Through intensive logging, the initial area has been reduced to around 12% of its original size. In this study we investigated the genetic variability and structure of the mountain lion, Puma concolor. Using 18 microsatellite loci we analyzed evidence of allele dropout, null alleles and stuttering, calculated the number of allele/locus, PIC, observed and expected heterozygosity, linkage disequilibrium, Hardy-Weinberg equilibrium, F IS, effective population size and genetic structure (MICROCHECKER, CERVUS, GENEPOP, FSTAT, ARLEQUIN, ONESAMP, LDNe, PCAGEN, GENECLASS software),we also determine whether there...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Araucaria Forest; Atlantic Rain Forest; Conservation; Genetic diversity; Microsatellite.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100009
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Molecular evidence for a recent demographic expansion in the puma (Puma concolor) (Mammalia, Felidae) Genet. Mol. Biol.
Matte,Eunice M.; Castilho,Camila S.; Miotto,Renata A.; Sana,Denis A.; Johnson,Warren E.; O'Brien,Stephen J.; Freitas,Thales R. O. de; Eizirik,Eduardo.
The puma is an iconic predator that ranges throughout the Americas, occupying diverse habitats. Previous phylogeographic analyses have revealed that it exhibits moderate levels of genetic structure across its range, with few of the classically recognized subspecies being supported as distinct demographic units. Moreover, most of the species' molecular diversity was found to be in South America. To further investigate the phylogeographic structure and demographic history of pumas we analyzed mtDNA sequences from 186 individuals sampled throughout their range, with emphasis on South America. Our objectives were to refine the phylogeographic assessment within South America and to investigate the demographic history of pumas using a coalescent approach. Our...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation genetics; Mammals; Mitochondrial DNA; Molecular time estimate; Phylogeography.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400018
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